From Tel-Aviv to Beer-Sheva:

Bus no. 370 (from central bus station)

Bus no. 380 (from central train station)

Train

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From Jerusalem to Beer-Sheva:

Bus no. 470

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From Beer-Sheva:

Buses no. 60, 64, 160 (to the gate)

Bus no. 65 (to the junction)

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Links:

Trains

Buses